Arterial neutral cholesteryl esterase. A hormone-sensitive enzyme distinct from lysosomal cholesteryl esterase.
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چکیده
منابع مشابه
Arterial neutral cholesteryl esterase. A hormone-sensitive enzyme distinct from lysosomal cholesteryl esterase.
We describe here an activable neutral cholesteryl esterase (EC 3.1.1.13) in arteries similar to the hormone-sensitive lipase of adipose tissue and adrenal cortex. Maximum enzyme activity in rabbit aorta was given by cholesteryl ester substrates dispersed as a mixed micelle with phosphatidylcholine and Na taurocholate (molar ratio 1:4:2). A quantitative assay of enzymic activity was obtained wit...
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Cholesteryl esterase activity has been demonstrated in the corpus luteum of the rat and the cow. The hydrolytic activity in bovine corpora lutea shows two pH optima, and is distributed throughout the particulate and supernatant fractions of the tissue. The greatest activity is present in the 5000 g pellet. The size of the available endogenous cholesteryl ester pools is also estimated. Some prop...
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A sensitive radioisotope microassay has been developed for the estimation of cholesteryl ester hydrolase (EC 3.1.1.13) in preparations of isolated rabbit aortic smooth muscle cells. Kinetic studies served to establish optimal assay conditions, which involve incubation in a total volume of 0.2 ml containing 5 pM cholesteryl oleate tritiated in the cholesterol moiety, 0.4 mM egg yolk lecithin, 0....
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The mechanisms of hydrolysis and accumulation of cholesteryl oleate-lipid droplets prepared in vitro were studied in acid cholesteryl-esterase-deficient fibroblasts (GM00863, GM03111). Acid cholesteryl esterase activity was reduced in both GM00863 and GM03111 (8.9% and 17.4% of the normal level, respectively), while neutral cholesteryl esterase activity was highly stimulated in GM03111. The hyd...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)33239-3